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Journal / Genetics / In Situ Labeling of Mitochondrial DNA Replication …
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Genetics

This Contenuto is Open Access.

JoVE Journal Genetics
In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
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Journal (Genetics)
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In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Article DOI: 10.3791/54516-v • 10:31 min • October 15th, 2016
October 15th, 2016 •
1, 1
1Lab of Molecular Genetics, National Heart, Lung and Blood Institute, National Institutes of Health

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Drosophila oogenesis continues to be exceptionally useful in the study of mitochondrial proliferation and inheritance. This manuscript describes a detailed protocol used to label the replicating mitochondrial DNA (mtDNA) in Drosophila adult ovaries with 5-ethynyl-2´-deoxyuridine (EdU), which facilitates uncovering mechanisms associated with mitochondrial inheritance that were previously debatable.

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In Situ Labeling Mitochondrial DNA Replication Drosophila Adult Ovaries EdU Staining Mitochondrial Field Mitochondrial Biogenesis Mitochondrial DNA Inheritance Structure Preservation Fluorescent Dye Penetration Drosophila Medium Dry Yeast Adult Female Flies Adult Males Carbon Dioxide Fly Pad Paint Brush Dissecting Pad Stereo Microscope Fine Nosed Forceps Lower Thorax Drosophila Medium Supplemented With 10%FBS Surrounding Gut And Extraneous Tissue Ovarioles
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