The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Antanas Spokevicius; Lynette Taylor; Emma Melder; Kim Van Beveren; Josquin Tibbits; Nicky Creux; Gerd Bossinger

doi:10.3791/54553

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L'uso di Induced somatica Settore Analisi (ISSA) per studio dei geni e promotori coinvolti nella formazione del legno e Sviluppo secondaria dello stelo

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

JoVE Journal
Genetica

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JoVE Journal Genetica

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

L'uso di Induced somatica Settore Analisi (ISSA) per studio dei geni e promotori coinvolti nella formazione del legno e Sviluppo secondaria dello stelo

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8,735 Views

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09:54 min

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October 05, 2016

DOI:

10.3791/54553-v

DOI:

10.3791/54553-v

•

09:54 min

October 05, 2016

•

4 Views

Antanas Spokevicius¹, Lynette Taylor¹, Emma Melder¹, Kim Van Beveren¹, Josquin Tibbits², Nicky Creux^3,4, Gerd Bossinger¹

¹School of Ecosystem and Forest Sciences, Faculty of Science,The University of Melbourne, ²Victorian AgriBiosciences Centre,La Trobe University R&D Park, ³College of Biological Sciences, Department of Plant Biology,University of California, Davis, ⁴Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI),University of Pretoria

Trascrizione

Summary

Here we present a protocol that facilitates the medium to high throughput functional characterization of gene and promoter constructs in tree secondary stem tissue within comparatively short time frames. It is efficient, easy to use and widely applicable to a range of tree species.