A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

Manuel Schmitz; Ben K. A. Nelemans; Theodoor H. Smit

doi:10.3791/54636

Rivista

/

Biologia dello sviluppo

/

Un sommerso Sandwich carta da filtro per il lungo termine Ex Ovo Time-lapse imaging di Chick primi embrioni

A Submerged Filter Paper Sandwich for Long-term <em>Ex Ovo</em> Time-lapse Imaging of Early Chick Embryos

JoVE Journal
Biologia dello sviluppo

This content is Free Access.

JoVE Journal Biologia dello sviluppo

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

Un sommerso Sandwich carta da filtro per il lungo termine Ex Ovo Time-lapse imaging di Chick primi embrioni

Please note that all translations are automatically generated. Click here for the English version.

22,138 Views

•

07:29 min

•

December 28, 2016

DOI:

10.3791/54636-v

DOI:

10.3791/54636-v

•

07:29 min

December 28, 2016

•

3 Views

Manuel Schmitz*¹, Ben K. A. Nelemans*¹, Theodoor H. Smit²

¹Department of Orthopaedic Surgery, VU University Medical Center Amsterdam,MOVE Research Institute Amsterdam, ²Department of Anatomy, Embryology & Physiology,Academic Medical Center Amsterdam

Trascrizione

Summary

Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique¹ that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.