Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Yeri Alice Rim; Yoojun Nam; Ji Hyeon Ju

doi:10.3791/54650

Rivista

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Biologia dello sviluppo

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Pluripotenti indotte generazione di cellule staminali da cellule del sangue Utilizzando Sendai Virus e centrifugazione

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Pluripotenti indotte generazione di cellule staminali da cellule del sangue Utilizzando Sendai Virus e centrifugazione

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14,172 Views

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09:57 min

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December 21, 2016

DOI:

10.3791/54650-v

DOI:

10.3791/54650-v

•

09:57 min

December 21, 2016

•

12 Views

Yeri Alice Rim¹, Yoojun Nam¹, Ji Hyeon Ju²

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine,The Catholic University of Korea

Trascrizione

Summary

We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.