Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Nicolaas H. Fourie; Ralph M. Peace; Sarah K. Abey; LeeAnne B. Sherwin; John W. Wiley; Wendy A. Henderson

doi:10.3791/54693

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Perturbazioni di circolazione miRNA nella sindrome dell'intestino irritabile rilevato utilizzando una piattaforma di espressione genica multiplex high-throughput

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

JoVE Journal
Genetica

This content is Free Access.

JoVE Journal Genetica

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbazioni di circolazione miRNA nella sindrome dell'intestino irritabile rilevato utilizzando una piattaforma di espressione genica multiplex high-throughput

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8,215 Views

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10:37 min

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November 30, 2016

DOI:

10.3791/54693-v

DOI:

10.3791/54693-v

•

10:37 min

November 30, 2016

•

9 Views

Nicolaas H. Fourie¹, Ralph M. Peace^1,2, Sarah K. Abey¹, LeeAnne B. Sherwin¹, John W. Wiley³, Wendy A. Henderson¹

¹Digestive Disorders Unit, National Institute of Nursing Research,National Institutes of Health, DHHS, ²National Institutes of Health Research Scholar,Howard Hughes Medical Institute, ³Internal Medicine, Medical School,University of Michigan

Trascrizione

Summary

We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.