A bassa densità primaria ippocampale Cultura Neuron
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17:46 min
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April 18, 2017
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Questo articolo descrive il protocollo per la coltura a bassa densità primari neuroni ippocampali crescono su vetrini invertiti su un monostrato gliale. Gli strati neuroni e gliali sono separati da perline di cera di paraffina. I neuroni coltivati con tale metodo adatto per imaging ottico ad alta risoluzione e saggi funzionali.
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Cite this Article
Roppongi, R. T., Champagne-Jorgensen, K. P., Siddiqui, T. J. Low-Density Primary Hippocampal Neuron Culture. J. Vis. Exp. (122), e55000, doi:10.3791/55000 (2017).
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