La differenziazione in vitro di cellule CD4 + umana FOXP3 + indotta da T regolatorie (iTregs) da cellule T CD4 Naïve utilizzando un protocollo TGF-β contenenti
20,405 Views
•
08:20 min
•
December 30, 2016
DOI:
Summary
Automatically generated
Questo protocollo descrive la generazione riproducibile e fenotipizzazione delle cellule T regolatorie indotte umane (iTregs) dalle cellule naïve T CD4 + in vitro. Diversi protocolli per l'induzione FOXP3 permettono lo studio di specifici fenotipi iTreg ottenuti con rispettivi protocolli.
Video correlati
14:01
Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
Video correlati
14529 Views
14:23
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Video correlati
24074 Views
09:27
New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
Video correlati
11208 Views
15:33
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Video correlati
15877 Views
12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Video correlati
34478 Views
07:12
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Video correlati
52017 Views
10:27
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
Video correlati
7213 Views
N/A
Generation of Human Regulatory T Cell Clones
Video correlati
1445 Views
07:46
In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
Video correlati
2954 Views
08:20
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Video correlati
20.4K Views
Read Article
Cite this Article
Schmidt, A., Éliás, S., Joshi, R. N., Tegnér, J. In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol. J. Vis. Exp. (118), e55015, doi:10.3791/55015 (2016).
Copy