Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / In vitro differentiatie van humane CD4 + FOXP3 +…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunology and Infection
In vitro differentiatie van humane CD4 + FOXP3 + geïnduceerde regulatoire T-cellen (iTregs) van naïeve CD4 + T cellen met behulp van een TGF-β-bevattende Protocol
Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
Journal (Immunology and Infection)
Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
Journal (Immunology and Infection)
Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
 
Click here for the English version

In vitro differentiatie van humane CD4 + FOXP3 + geïnduceerde regulatoire T-cellen (iTregs) van naïeve CD4 + T cellen met behulp van een TGF-β-bevattende Protocol

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Dit protocol beschrijft de reproduceerbare productie en fenotypering van mens veroorzaakte regulerende T-cellen (iTregs) van naïeve CD4 + T-cellen in vitro. Verschillende protocollen voor FOXP3 inductie mogelijk maken voor de studie van specifieke iTreg fenotypes verkregen met de respectieve protocollen.

Tags

Immunologie regulatoire T cellen Treg CD4 + T-cellen magnetische celisolatie, cytokines FOXP3 TGF-β IL-2 intracellulaire Flowcytometrie qRT-PCR
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter