Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Daniel von Wangenheim; Robert Hauschild; Ji&#345;&#237; Friml

doi:10.3791/55044

Rivista

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Biologia

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Luce Foglio microscopia a fluorescenza delle radici delle piante che crescono sulle superficie di un gel

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

JoVE Journal
Biologia
Author Produced

This content is Free Access.

JoVE Journal Biologia

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Luce Foglio microscopia a fluorescenza delle radici delle piante che crescono sulle superficie di un gel

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14,603 Views

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06:41 min

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January 18, 2017

DOI:

10.3791/55044-v

DOI:

10.3791/55044-v

•

06:41 min

January 18, 2017

•

4 Views

Daniel von Wangenheim¹, Robert Hauschild², Jiří Friml¹

¹Developmental and Cell Biology of Plants,Institute of Science and Technology Austria, ²Bioimaging Facility,Institute of Science and Technology Austria

Trascrizione

Summary

This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.