Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Joni H. Ylostalo; Nikolay Bazhanov; Arezoo Mohammadipoor; Thomas J. Bartosh

doi:10.3791/55126

Produzione e somministrazione di Therapeutic staminali mesenchimali / stromali cellulare (MSC) Sp...

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JoVE Journal Developmental Biology

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Produzione e somministrazione di Therapeutic staminali mesenchimali / stromali cellulare (MSC) Spheroids innescato in Culture 3-D, in condizioni Xeno-free

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11:05 min

March 18, 2017

DOI: 10.3791/55126-v

Joni H. Ylostalo¹, Nikolay Bazhanov², Arezoo Mohammadipoor³, Thomas J. Bartosh⁴

¹Department of Biology,University of Mary Hardin-Baylor, ²Department of Pediatrics,University of Texas Medical Branch, ³Multi-Organ Support Technology Task Area,U.S. Army Institute of Surgical Research, ⁴Internal Medicine,Texas A&M University Health Science Center

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Overview

This article discusses the generation and administration of therapeutic spherical aggregates, or spheroids, of mesenchymal stem/stromal cells (MSCs) primed under xeno-free conditions. These methods aim to enhance the efficacy of MSC therapeutics by addressing challenges related to cell retention and functionality post-transplantation.

Key Study Components

Area of Science

Stem Cell Therapy
Regenerative Medicine
Tissue Engineering

Background

MSCs have significant therapeutic potential.
Current methods of cell preparation for clinical use are debated.
Dispersed cell transplantation often leads to poor outcomes.
Xeno-free conditions can improve clinical applicability.

Purpose of Study

To develop protocols for generating MSC spheroids.
To enhance cell survival and functionality post-transplantation.
To eliminate the use of immunogenic animal products in MSC preparation.

Methods Used

Preparation of MSC spheroids under xeno-free conditions.
Injection protocols for therapeutic application.
Evaluation of cell retention and functionality.
Comparison of outcomes with traditional dispersed cell methods.

Main Results

MSC spheroids showed improved retention and functionality.
Elimination of animal products enhanced clinical relevance.
Protocols demonstrated potential for both experimental and clinical applications.
Findings may inform future MSC-based therapies.

Conclusions

MSC spheroids represent a promising approach in regenerative medicine.
Protocols developed can facilitate better therapeutic outcomes.
Further research is needed to optimize these methods.

Frequently Asked Questions

What are mesenchymal stem/stromal cells?

MSCs are multipotent stem cells that can differentiate into various cell types and are used in regenerative medicine.

Why are xeno-free conditions important?

Xeno-free conditions reduce the risk of immune reactions and improve the safety of cell therapies.

What are the advantages of MSC spheroids?

MSC spheroids enhance cell retention, survival, and functionality compared to dispersed cells.

How can these methods impact clinical applications?

By improving the efficacy of MSC therapies, these methods can lead to better patient outcomes.

Who conducted the study?

The study was conducted by technicians Josh Beaver and Bret Clough from the laboratory.

What challenges do MSC therapies face?

Challenges include poor cell retention, survival, and functionality after transplantation.

What is the future direction of this research?

Future research will focus on optimizing MSC spheroid protocols for broader clinical applications.

Il potenziale terapeutico delle cellule staminali/stromali mesenchimali (MSC) è ben documentato, tuttavia il metodo migliore per preparare le cellule per i pazienti rimane controverso. In questo articolo, comunichiamo i protocolli per generare e somministrare in modo efficiente aggregati sferici terapeutici o "sferoidi" di MSC innescati in condizioni prive di xeno per applicazioni sperimentali e cliniche.

L'obiettivo generale di questi esperimenti è quello di generare e iniettare in modo efficiente aggregati sferici terapeutici, o sferoidi, di cellule staminali/stromali mesenchimali, o MSC, innescate in condizioni prive di xeno in applicazioni sperimentali e cliniche. Questi metodi possono aiutare a superare le principali barriere nel campo delle terapie MSC, come la scarsa ritenzione cellulare, la sopravvivenza e la funzionalità. Questo è spesso visto dopo il trapianto di cellule disperse e in condizioni avverse comunemente associate a lesioni tissutali.

Il vantaggio principale di queste procedure è che gli sferoidi MSC possono essere preparati, innescati e iniettati in assenza di prodotti animali immunogenici, come l'FBS, che attualmente limita alcune applicazioni cliniche delle cellule e può anche confondere l'interpretazione di alcuni dati preclinici. Sebbene questo meccanismo sia potenzialmente molto utile nel mediare l'efficacia basata sulle MSC, può anche essere utilizzato per comprendere la riparazione tissutale mediata dalle MSC e anche come le altre formulazioni di terreni possono modificare il genex plus terapeutico. A dimostrare queste procedure saranno Josh Beaver e Bret Clough, due tecnici del mio laboratorio.

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