Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Evaluation of Intracellular Location of Reactive…
JoVE Journal
Developmental Biology
Author Produced

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Developmental Biology
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
Journal (Developmental Biology)
FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
Journal (Developmental Biology)
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
 

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Article DOI: 10.3791/55323-v • 10:24 min • March 11th, 2018
March 11th, 2018 •
1,2, 2,3
1Department of Molecular Biology, University of León, 2INDEGSAL, University of León, 3Planta de Cultivos el Bocal, Spanish Institute of Oceanography (IEO)

Capitoli

Riepilogo
Automatic Translation

This protocol describes a detailed methodology for detecting H2O2 localization within Solea senegalensis spermatozoa using a sensitive fluorochrome DCFH-DA for ROS, a live mitochondria stain for mitochondria, and DAPI for nuclei visualization, respectively. The protocol is designed to be performed within 2 h with either fresh or thawed spermatozoa.

Tags

Intracellular Location Reactive Oxygen Species Solea Senegalensis Spermatozoa Confocal Microscopy Oxidative Stress Sperm Function Cellular Damage Motility Fertilization Lipid Peroxidation Apoptotic Escape Peroxide Mitochondria Nucleus DNA Fluorophore Green Fluorescence Protocol Commercial Interest Sibrifis Mirica Physically-reactive Biology Apiculture Fields Cryopreservation Sperm Activation Breathers
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter