Un saggio di HS-MRM per la quantificazione delle proteine di cellula ospite in biofarmaceutica di proteine mediante spettrometria di massa di liquido cromatografia dello ione mobilità QTOF
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April 17, 2018
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Qui, descriviamo un'analisi cromatografica accoppiata con la separazione di mobilità dello ione dei precursori del peptide seguita dalla ad alta risoluzione (~ 30.000) MS-rilevazione dei frammenti peptidici per la quantificazione degli standard del peptide a spillo in un anticorpo monoclonale digest.
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Cite this Article
Doneanu, C., Fang, J., Alelyunas, Y., Yu, Y. Q., Wrona, M., Chen, W. An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry. J. Vis. Exp. (134), e55325, doi:10.3791/55325 (2018).
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