Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Christiane Grimm; Johannes Vierock; Peter Hegemann; Jonas Wietek

doi:10.3791/55497

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Registrazioni patch-clamp intere cellule per la determinazione elettrofisiologica della selettività ionica in Channelrhodopsins

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Registrazioni patch-clamp intere cellule per la determinazione elettrofisiologica della selettività ionica in Channelrhodopsins

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17,005 Views

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08:39 min

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May 22, 2017

DOI:

10.3791/55497-v

DOI:

10.3791/55497-v

•

08:39 min

May 22, 2017

•

6 Views

Christiane Grimm*¹, Johannes Vierock*¹, Peter Hegemann¹, Jonas Wietek¹

¹Experimental Biophysics, Institute of Biology,Humboldt-Universität zu Berlin

Trascrizione

Summary

This article describes how the ion selectivity of channelrhodopsin is determined with electrophysiological whole-cell patch-clamp recordings using HEK293 cells. Here, the experimental procedure for investigating chloride selectivity of an anion-selective channelrhodopsin is demonstrated. However, the procedure is transferable to other channelrhodopsins of distinct selectivity.