Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Jonas Gross; Nicolas Wirth; Ingrid Tessmer

doi:10.3791/55501

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Microscopia a forza atomica Ricerche del riconoscimento delle lesioni del DNA nella riparazione di excisione dei nucleotidi

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

JoVE Journal
Genetica

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JoVE Journal Genetica

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Microscopia a forza atomica Ricerche del riconoscimento delle lesioni del DNA nella riparazione di excisione dei nucleotidi

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9,346 Views

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10:59 min

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May 24, 2017

DOI:

10.3791/55501-v

DOI:

10.3791/55501-v

•

10:59 min

May 24, 2017

•

1 Views

Jonas Gross*¹, Nicolas Wirth*¹, Ingrid Tessmer¹

¹Rudolf Virchow Center for Experimental Biomedicine,University of Würzburg

Trascrizione

Summary

Here, the study of different DNA lesion recognition approaches via single molecule AFM imaging is demonstrated with the nucleotide excision repair system as an example. The procedures of DNA and protein sample preparations and experimental as well as analytical details for the AFM experiments are described.