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Biochemistry

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Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Article DOI: 10.3791/55932-v • 12:55 min • October 10th, 2017
October 10th, 2017 •
1,2, 1,2, 1,2
1Systems Biotechnology Research Center, KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology

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This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.

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Assay Phosphorylation Microtubule Binding Tau Protein Colorectal Cancer Cells Confocal Microscopy Biological Research Cancer Research Dementia Research Phosphatase Assay Microtubule-binding Assay Tau Localization Technique Antibodies HCT 116 Cells Culture Dishes Minimum Essential Medium Fetal Bovine Serum Penicillin Streptomycin Curcumin Lithium Chloride
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