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Immunology and Infection

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Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Article DOI: 10.3791/55949-v • 12:48 min • August 21st, 2017
August 21st, 2017 •
1, 1, 1, 1
1Laboratory of Physiological Chemistry, Faculty of Pharmacy, Takasaki University of Health and Welfare

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The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.

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Purification Membrane Compartment Endoplasmic Reticulum-associated Degradation Exogenous Antigens Cross-presentation Immunity Period Cellular Compartment ERAD-like Degradation Purified Vesicles Molecular Operations Insight Dendritic Cells Antigen-presenting Cells Non-specific Background DC2.4 Cells RPMI Buffer Biotinylated Ovalbumin Exogenous Antigen Incubate Harvest Cells Centrifuge Processing Presenting
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