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JoVE Journal Developmental Biology
Herprogrammering van de primaire vruchtwater en membraan cellen pluripotent in Xeno-vrij voorwaarden
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Herprogrammering van de primaire vruchtwater en membraan cellen pluripotent in Xeno-vrij voorwaarden

Article DOI: 10.3791/56003-v • 09:34 min • November 27th, 2017
November 27th, 2017 •
1, 2, 1, 3, 3,4,5, 1, 1
1Mitchell Cancer Institute, University of South Alabama, 2College of Medicine, University of South Alabama, 3Institute for Regenerative Medicine, University of Zurich, 4Department of Dermatology, University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus

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Dit protocol beschrijft de herprogrammering van de primaire vruchtwater vloeistof en membraan mesenchymale stamcellen in geïnduceerde pluripotente stamcellen met gebruikmaking van een niet-integratie van episomal in volledig chemisch gedefinieerde voorwaarden. Procedures van extractie, cultuur, herprogrammering en karakterisering van de resulterende geïnduceerde pluripotente stamcellen door strenge methoden zijn gedetailleerd.

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Ontwikkelingsbiologie kwestie 129 iPSC vruchtwater amnion herprogrammering episomal E8 middellange vitronectin
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