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Neuroscience

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JoVE Journal Neuroscience
Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons
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Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

Article DOI: 10.3791/56026-v • 07:31 min • September 7th, 2017
September 7th, 2017 •
1, 2, 1,2, 1
1Department of Biology and Biotechnology, Worcester Polytechnic Institute, 2Department of Biomedical Engineering, Worcester Polytechnic Institute

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The use of an adapted "olfactory chip" for the efficient calcium imaging of C. elegans males is described here. Studies of male exposure to glycerol and a pheromone are also shown.

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Microfluidic Olfactory Chip Neuronal Activity Imaging Pheromones Male C. Elegans Calcium Transience Neurobiology Neural Circuit Regulation Micro-fluidic Trap Device Loading Animals Biogenic Pheromones Reservoir Assembly Tubing Preparation Buffer Preparation Fluorescin Air Bubbles Removal Micro-fluidic Device Inlet Port
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