Elettroforesi gel orizzontale per la rilevazione avanzata dei complessi proteina-RNA
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July 28, 2017
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Summary
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L'elettroforesi natale di poliacrilammide è uno strumento fondamentale per analizzare le interazioni tra proteine RNA. Tradizionalmente la maggior parte degli esperimenti hanno utilizzato gel verticale. Tuttavia, i gel orizzontali offrono diversi vantaggi, come l'opportunità di monitorare i complessi durante l'elettroforesi. Forniamo un protocollo dettagliato per la generazione e l'uso di elettroforesi geotermica orizzontale.
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Cite this Article
Dowdle, M. E., Imboden, S. B., Park, S., Ryder, S. P., Sheets, M. D. Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes. J. Vis. Exp. (125), e56031, doi:10.3791/56031 (2017).
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