Stereologiche stima del numero di neuroni dopaminergici nel Nigra di Substantia del Mouse utilizzando il frazionatore ottica e microscopia Standard attrezzature
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September 01, 2017
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Questo lavoro presenta un protocollo passo-passo per la valutazione imparziale stereologiche dei numeri di un neurone delle cellule dopaminergiche nel nigra di substantia del mouse utilizzando attrezzature di microscopia standard (cioè, un microscopio ottico, una tabella di oggetto motorizzato (x, y, z aereo) e software di pubblico dominio per analisi digitale delle immagini.
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Ip, C. W., Cheong, D., Volkmann, J. Stereological Estimation of Dopaminergic Neuron Number in the Mouse Substantia Nigra Using the Optical Fractionator and Standard Microscopy Equipment. J. Vis. Exp. (127), e56103, doi:10.3791/56103 (2017).
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