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Journal / Biochemistry / Quantification of the Abundance and Charging…
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Biochemistry

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Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Article DOI: 10.3791/56212-v • 10:34 min • August 22nd, 2017
August 22nd, 2017 •
1, 1, 1
1Department of Biology, University of Copenhagen

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Here we present a method for directly measuring transfer RNA charging levels from purified Escherichia coli RNA as well as a way to compare relative levels of transfer RNA, or any other short RNA, across different samples based on the addition of spike-in cells expressing a reference gene.

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Quantification Abundance Charging Levels Transfer RNAs Escherichia Coli Experiment Cell Biology Growth States RNA Levels Composition Spike-in Culture TRNA Cell C IPTG OD436 Harvesting TCA Enzymatic Activities
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