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Capitoli
- 00:05Titolo
- 01:00GSD Super-resolution Imaging Photoswiching Buffer Preparation
- 02:22Mounting of the Samples
- 02:57Image Acquisition
- 04:38Image Analysis
- 06:07Results: Super-resolution GSD Imaging Improves Resolution
- 07:08Conclusion
This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.
