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Journal / Bioengineering / Metabolic Support of Excised, Living Brain…
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Bioengineering

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JoVE Journal Bioengineering
Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition
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Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition

Article DOI: 10.3791/56282-v • 10:21 min • October 18th, 2017
October 18th, 2017 •
1,2, 2,3, 4, 2,3,5, 1,2,6
1Department of Neuroscience, University of Florida, 2McKnight Brain Institute, University of Florida, 3Department of Biomedical Engineering, University of Florida, 4Center for Functionally Integrative Neuroscience, Aarhus University, 5Department of Radiology, University of Florida, 6National High Magnetic Field Laboratory, Florida State University

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The current protocol describes a method by which users can maintain viability of acute hippocampal and cortical slice preparations during the collection of magnetic resonance microscopy data.

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Metabolic Support Excised Brain Tissues Magnetic Resonance Microscopy Living Tissue Samples MR Contrast Properties Medical Imaging Perfusate Conditions Continuous Flow Coil Probe Assembly Oxygenator Hardware Artificial Cerebrospinal Fluid (aCSF) Equilibrating ACSF Carbogen Saturation PH Measurement Dissolved Oxygen Content Tissue Metabolism Priming Procedure
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