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Genetics

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JoVE Journal Genetics
Generation av en gen-stört Streptococcus mutans stam utan genen kloning
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Generation av en gen-stört Streptococcus mutans stam utan genen kloning

Article DOI: 10.3791/56319-v • 12:06 min • October 23rd, 2017
October 23rd, 2017 •
1, 1, 1,2,3, 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

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Vi beskriver en lättköpt, snabba och relativt billig metod för generering av gen-stört Streptococcus mutans stammar; Denna teknik kan anpassas för generering av gen-stört stammar av olika arter.

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Genetik fråga 128 Gene störningar DNA konstruera polymeras-kedjereaktion primer design markörgen för antibiotikaresistens Streptococcus mutans elektroporation homolog rekombination
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