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Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
DOI:

12:06 min

February 04, 2018

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Capitoli

  • 00:00Titolo
  • 01:42Cell seeding and pulse labeling of the SNAP-tag
  • 04:15Time-lapse microscopy
  • 05:23Image processing and analysis
  • 07:55Risultati
  • 10:13Conclusions

Summary

Traduzione automatica

This protocol describes a method to determine protein half-lives in single living adherent cells, using pulse labeling and fluorescence time-lapse imaging of SNAP-tag fusion proteins.

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