Isolamento e misure respiratoria dei mitocondri da Arabidopsis thaliana
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January 05, 2018
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Come i mitocondri sono solo una piccola percentuale della cellula vegetale, hanno bisogno di essere purificato per una serie di studi. I mitocondri possono essere isolati da una varietà di organi vegetali di omogeneizzazione, seguita da centrifugazione in gradiente di differenziale e densità per ottenere una frazione altamente purificata mitocondriale.
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Lyu, W., Selinski, J., Li, L., Day, D. A., Murcha, M. W., Whelan, J., Wang, Y. Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana. J. Vis. Exp. (131), e56627, doi:10.3791/56627 (2018).
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