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En konstruerad Split-TET2 enzym för kemisk-inducerbara DNA Hydroxymethylation och epigenetiska Remodeling
Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
Journal (Chemistry)
Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
Journal (Chemistry)
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En konstruerad Split-TET2 enzym för kemisk-inducerbara DNA Hydroxymethylation och epigenetiska Remodeling

Article DOI: 10.3791/56858-v • 08:34 min • December 18th, 2017
December 18th, 2017 •
1, 2, 1
1Centre for Epigenetics and Disease Prevention, Department of Molecular and Cellular Medicine, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2Centre for Translational Cancer Research, Department of Medical Physiology, Institute of Biosciences and Technology, College of Medicine, Texas A&M University

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Detaljerade protokoll för att införa ett bakåtkompilerade split-TET2 enzym (CiDER) i däggdjursceller för kemiska inducerbara DNA hydroxymethylation och epigenetiska remodeling presenteras.

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Kemi fråga 130 DNA-metylering epigenetik kromatin tillgänglighet DNA demetylering kemisk biologi TET2 5-hydroxymethylcytosine transkription genuttryck epigenomet redigering
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