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Journal / Cancer Research / Genome-wide RNAi Screening att identifiera värd…
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Cancer Research

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JoVE Journal Cancer Research
Genome-wide RNAi Screening att identifiera värd faktorer som modulerar onkolytisk Virus terapi
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Genome-wide RNAi Screening att identifiera värd faktorer som modulerar onkolytisk Virus terapi

Article DOI: 10.3791/56913-v • 08:51 min • April 3rd, 2018
April 3rd, 2018 •
1,2, 1,4, 1, 1, 1,2,3
1Children's Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology, University of Ottawa, 3Department of Pediatrics, University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary

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Här beskriver vi ett protokoll för att anställa hög genomströmning RNAi screening att avslöja värd mål som kan manipuleras för att förbättra onkolytisk virus behandling, särskilt rhabodvirus och vaccinia virus terapi, men det kan lätt anpassas till andra onkolytisk virus-plattformar eller för att upptäcka värd gener som modulera virusreplikation generellt.

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Cancerforskning fråga 134 onkolytisk virus RNAi skärm hög genomströmning cancer vaccinia rhabdovirus cell värd funktionsgenomik
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