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Journal / Developmental Biology / Klonede analyse af embryonale hæmatopoietisk…
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Developmental Biology

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JoVE Journal Developmental Biology
Klonede analyse af embryonale hæmatopoietisk stamcelle prækursorer ved hjælp af enkelt celle indeks sortering kombineret med endotel celle Niche Co kultur
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Klonede analyse af embryonale hæmatopoietisk stamcelle prækursorer ved hjælp af enkelt celle indeks sortering kombineret med endotel celle Niche Co kultur

Article DOI: 10.3791/56973-v • 09:32 min • May 8th, 2018
May 8th, 2018 •
1,2, 1, 1, 1, 1,2
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Washington School of Medicine

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Her præsenterer vi metode til klonede analyse af hæmatopoietisk stamcelle prækursorer under murine embryonale udvikling. Vi kombinere indeks sortering af enkelt celler fra embryonale aorta-gonade-mesonephros regionen med endotel Co cellekultur og transplantation til at karakterisere fænotypiske egenskaber og engraftment potentiale af enkelt hæmatopoietisk prækursorer.

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Udviklingsmæssige biologi spørgsmålet 135 hæmatopoietisk stamcelle (HSC) pre-HSC aorta-gonade-mesonephros region (AGM) embryonale bloddannelsen indeks sortering enkelt celle klonede analyse hæmatopoietisk stamcelletransplantation
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