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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Article DOI: 10.3791/56981-v • 12:04 min • October 3rd, 2018
October 3rd, 2018 •
1, 2, 2, 3, 3, 3, 4, 1, 4, 5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy

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The protocols described allow the construction, characterization and selection (against the target of choice) of a "domainome" library made from any DNA source. This is achieved by a research pipeline that combines different technologies: phage display, a folding reporter and next generation sequencing with a web tool for data analysis.

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Interactome-Seq Protocol Domainome Library Construction Validation Selection Phage Display Next Generation Sequencing Protein Research Annotation Protein Domains Structural Properties Functional Properties Protein Interaction Networks Antigen Antibody Signatures Unbiased Technique High Throughput Modular Study Single Gene Whole Genome Functional Studies Phage Display Context Selection Against Different Targets Binding Proteins Purified Antibodies NGS Analysis Web Tools Development ORF Library Construction
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