Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Laminar Flow-based Assays to Investigate…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Immunology and Infection
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
Journal (Immunology and Infection)
Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
Journal (Immunology and Infection)
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
 

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Article DOI: 10.3791/57009-v • 08:50 min • April 9th, 2018
April 9th, 2018 •
1, 1, 1, 2, 1, 1, 1
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine

Capitoli

Riepilogo
Automatic Translation

Leukocytes avidly interact with vascular cells and platelets after vessel wall injury or during inflammation. Here, we describe a straightforward laminar flow-based assay to characterize the molecular mechanisms that underlie the interactions between leukocytes and their cellular partners.

Tags

Laminar Flow-based Assays Leukocyte Recruitment Cultured Vascular Cells Adherent Platelets Immunology Inflammation Cost Effective User Friendly Experimental Conditions Leukocyte Adhesion Tumor Cells Immobilized Cells Substrates Annemiek Dickhout Engineer PhD Student Blood Samples Acid Citrate Dextrose Centrifuge Platelet-rich Plasma Platelet-poor Plasma Platelet Buffer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter