Ottimizzando la costituzione genetica di chimico sonde in GPCR per foto-reticolazione Mapping e chimica di Bioorthogonal in cellule di mammiferi dal vivo
8,449 Views
•
14:02 min
•
April 09, 2018
DOI:
Summary
Automatically generated
Un'analisi di fluorescenza facile è presentata per valutare l'efficienza di amino-acil-tRNA-sintetasi/tRNA coppie conglobando amino acidi non canonico (ncAAs) proteine espresse nelle cellule di mammifero. L'applicazione di ncAAs per studiare i recettori accoppiati a proteine G (GPCR) è descritto, tra cui la mappatura foto-reticolazione di associazione siti e bioorthogonal GPCR etichettatura su cellule vive.
Video correlati
10:49
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
Video correlati
12608 Views
14:43
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
Video correlati
10806 Views
07:59
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
Video correlati
7664 Views
08:21
Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
Video correlati
6842 Views
14:12
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Video correlati
5184 Views
08:46
A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
Video correlati
2178 Views
14:26
In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
Video correlati
13222 Views
09:40
Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
Video correlati
17957 Views
09:20
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
Video correlati
2340 Views
16:16
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Video correlati
15204 Views
Read Article
Cite this Article
Serfling, R., Seidel, L., Böttke, T., Coin, I. Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells. J. Vis. Exp. (134), e57069, doi:10.3791/57069 (2018).
Copy