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En enkel och effektiv metod för In Vivo hjärt-specifik gen Manipulation av Intramyocardial injektion i möss
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En enkel och effektiv metod för In Vivo hjärt-specifik gen Manipulation av Intramyocardial injektion i möss

Article DOI: 10.3791/57074-v • 06:42 min • April 16th, 2018
April 16th, 2018 •
*1,2, *2, *2, 1, 1, 1, 1,2
1Department of Cardiology, The First Affiliated Hospital of Bengbu Medical College, 2Department of Cardiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University
* These authors contributed equally

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Här presenterar vi ett protokoll för manipulering av hjärt-specifika genen i möss. Under anestesi, var mus hjärtan externalized via den fjärde interkostalrummet. Därefter blot adenoviruses kodning specifika gener skulle injiceras med en spruta i myokardiet, följt av protein uttryck mätning via i vivo imaging och Western analys.

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Biologi fråga 134 medicin hjärt- mus gen överuttryck överväldigande hjärtat
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