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Developmental Biology

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JoVE Journal Developmental Biology
Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea
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Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea

Article DOI: 10.3791/57168-v • 06:14 min • March 26th, 2018
March 26th, 2018 •
1, 1, 1
1Department of Molecular Medicine, College of Veterinary Medicine, Cornell University

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The planarian Schmidtea mediterranea is an excellent model for studying stem cells and tissue regeneration. This publication describes a method to selectively remove one organ, the pharynx, by exposing animals to the chemical sodium azide. This protocol also outlines methods for monitoring pharynx regeneration.

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Chemical Amputation Regeneration Pharynx Planarian Schmidtea Mediterranea Stem Cells Sodium Azide Precise Technique Organ Damage Visual Demonstration Divya Shiroor Graduate Student Plastic Transfer Pipette Petri Dish Ruler Length Measurement Stereo Microscope
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