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Journal / Immunology and Infection / आदेशित, अनावश्यक लायब्रेरी की प्रतिकृति…
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Immunology and Infection

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JoVE Journal Immunology and Infection
आदेशित, अनावश्यक लायब्रेरी की प्रतिकृति Pseudomonas aeruginosa तनाव PA14 Transposon लन म्यूटेंट
Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Journal (Immunology and Infection)
Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
Journal (Immunology and Infection)
Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
 
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आदेशित, अनावश्यक लायब्रेरी की प्रतिकृति Pseudomonas aeruginosa तनाव PA14 Transposon लन म्यूटेंट

Article DOI: 10.3791/57298-v • 11:35 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 1, 1, 2,3, 1,4, 1,4
1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School
* These authors contributed equally

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Pseudomonas aeruginosa संक्रमण कमजोर मेजबान में महत्वपूर्ण रुग्णता का कारण बनता है । पी aeruginosa तनाव PA14 के निरर्थक transposon प्रविष्टि उत्परिवर्ती पुस्तकालय, PA14NR सेट के रूप में नामित, कई प्रक्रियाओं में जीन की कार्यक्षमता के विश्लेषण की सुविधा । यहां प्रस्तुत एक PA14NR सेट उत्परिवर्ती पुस्तकालय के उच्च गुणवत्ता प्रतियां उत्पंन प्रोटोकॉल है ।

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इम्यूनोलॉजी और संक्रमण अंक १३५ Pseudomonas aeruginosa मेरिनर transposon उत्परिवर्ती पुस्तकालय mutagenesis सूक्ष्म जीव विज्ञान अवसरवादी संक्रमण इम्यूनोलॉजी
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