Procedura di RNA Pull-down per identificare gli obiettivi di RNA di un RNA lunghi Non codificanti
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09:36 min
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April 10, 2018
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Questo metodo di discesa di RNA permette di identificare i bersagli di RNA di un RNA lunghi non codificanti (lncRNA). Basato sull'ibridazione di fatto in casa, progettato anti-senso del DNA del oligonucleotide sonde specifiche per questo lncRNA in un tessuto opportunamente fisso o linea cellulare, in modo efficiente permette la cattura di tutti i target di RNA della lncRNA.
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Torres, M., Becquet, D., Guillen, S., Boyer, B., Moreno, M., Blanchard, M., Franc, J., François-Bellan, A. RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA. J. Vis. Exp. (134), e57379, doi:10.3791/57379 (2018).
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