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Developmental Biology

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Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

Article DOI: 10.3791/57527-v • 08:17 min • June 1st, 2018
June 1st, 2018 •
1,2, 3, 1,2
1Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, 2Caruso Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine of the University of Southern California, 3Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University

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Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.

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Three-dimensional Organotypic Cultures Vestibular Sensory Organ Auditory Sensory Organ Inner Ear Research Mechanical Force Tissue Stiffness Molecular Signaling Inner Ear Development Dr. Hudspeth Morphysiologic Conditions In-vitro Growth 70% Ethanol Dissection Instruments Number Five Forceps Hair Knife Sterile Scalpel Blade Longitudinal Cut Split The Head Of The Mouse Extract The Temporal Bones HBSS Buffer Petri Dish Ice-cold HBSS Buffer Utricle
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