Metodologia per la rilevazione accurata di metilazione del DNA mitocondriale
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May 20, 2018
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Qui, presentiamo un protocollo per consentire la quantificazione accurata della metilazione del DNA (mtDNA) mitocondriale. In questo protocollo, descriviamo una digestione enzimatica del DNA con BamHI accoppiato con una pipeline di analisi bioinformatica che può essere utilizzata per evitare la sopravvalutazione dei livelli di metilazione del DNA mitocondriale causato dalla struttura secondaria del mtDNA.
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Cite this Article
Mechta, M., Ingerslev, L. R., Barrès, R. Methodology for Accurate Detection of Mitochondrial DNA Methylation. J. Vis. Exp. (135), e57772, doi:10.3791/57772 (2018).
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