Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Contrast-Matching Detergent in Small-Angle…
JoVE Journal
Biochemistry

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Biochemistry
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Journal (Biochemistry)
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
Journal (Biochemistry)
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
 

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Article DOI: 10.3791/57901-v • 10:27 min • October 21st, 2018
October 21st, 2018 •
1, 2, 1, 1, 2, 1
1Neutron Scattering Division, Oak Ridge National Laboratory, 2School of Chemistry and Biochemistry, Georgia Institute of Technology

Capitoli

Riepilogo
Automatic Translation

This protocol demonstrates how to obtain a low-resolution ab initio model and structural details of a detergent-solubilized membrane protein in solution using small-angle neutron scattering with contrast-matching of the detergent.

Tags

Contrast-matching Detergent Small-angle Neutron Scattering Membrane Protein Structural Analysis Ab Initio Modeling Integral Membrane Proteins Oligomeric State Overall Shape Low Resolution Structure Solution Studies Stabilizing Molecules Invisible Detergents Signal From Membrane Protein Biological Research Medical Research Deuterium Labeling Neutron Scattering Length Densities SLDs Contrast Variation Data Analysis MULCh Web Application Project Title Subunit One Subunit Two Molecular Formula Volume In Cubic Angstroms Buffer Components Neutron Contrast Calculation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter