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Immunology and Infection

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Generation av Knock-out primära och utökade mänskliga NK-celler använder Cas9 Ribonucleoproteins

Article DOI: 10.3791/58237-v • 07:26 min • June 14th, 2018
June 14th, 2018 •
1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 3, 1
1Center for Childhood Cancer and Blood Disease, Nationwide Children's Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, 3Center for Clinical and Translational Research, Nationwide Children's Hospital Research Institute

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Här presenterar vi ett protokoll för att genetiskt modifiera primär eller utökad mänskliga naturliga mördarceller (NK) celler använder Cas9 Ribonucleoproteins (Cas9/RNPs). Med hjälp av detta protokoll, genererade vi mänskliga NK celler bristfällig för omvandla tillväxtfaktor – b receptor 2 (TGFBR2) och hypoxantin phosphoribosyltransferase 1 (HPRT1).

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Immunologi och infektion fråga 136 mänskliga primära NK celler mänskliga expanderat NK-celler CRISPR/Cas9 Cas9/RNPs gRNAs TGFBR2 TGFβ resistenta NK-celler
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