Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Dandan Li; H&#243;ngyi Zh&#224;o; Wei Wei; Nan Liu; Yonghua Dr. Huang

doi:10.3791/58423

Sub-acuta microemorragie cerebrale indotta tramite l'iniezione del Lipopolysaccharide in ratti

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Neuroscience

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JoVE Journal Neuroscience

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Sub-acuta microemorragie cerebrale indotta tramite l'iniezione del Lipopolysaccharide in ratti

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06:39 min

October 17, 2018

DOI: 10.3791/58423-v

Dandan Li*^1,2, Hóngyi Zhào*^2,3, Wei Wei², Nan Liu², Yonghua Dr. Huang²

¹Department of Neurology,Second Hospital of Shanxi Medical University, ²Department of Neurology,PLA Army General Hospital, ³Department of Neurology,NO 261 Hospital of PLA

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Overview

This study presents a protocol for inducing and detecting cerebral micro-hemorrhages (CMHs) in Sprague-Dawley rats through LPS injection. The work contributes to understanding the pathogenesis of CMHs and may inform future research into various brain disorders.

Key Study Components

Area of Science

Neuroscience
Pathophysiology
Animal Models

Background

Cerebral micro-hemorrhages are associated with various neurological disorders.
Inflammation plays a critical role in the development of CMHs.
LPS is commonly used to induce inflammation in experimental models.
The technique may extend to studying other brain disorders like Parkinson's and Alzheimer's disease.

Purpose of Study

To develop a reliable method for inducing CMHs in a laboratory setting.
To explore the inflammatory processes involved in CMH pathogenesis.
To establish a basis for future research on brain disorders.

Methods Used

LPS is administered via intraperitoneal injection at a dose of 1 mg/kg.
The animal model used is 10-week-old male Sprague-Dawley rats.
Cardiac puncture and Evans blue dye are used for assessing blood-brain barrier integrity.
The protocol includes multiple injections and specific tissue preparation steps for imaging.
Various staining techniques are applied for analyzing brain tissue.

Main Results

The LPS injection reliably induces CMHs, observable through various imaging techniques.
Methods allow the visualization of red blood cells outside blood vessels via H&E staining, indicating CMH presence.
Evans blue staining indicates compromised blood-brain barrier function and highlights CMH distribution.

Conclusions

This study demonstrates a straightforward method for investigating CMHs and their underlying mechanisms.
Insights gained could enhance understanding of CMHs and related neurological disorders.
The protocol also enables researchers to examine inflammation's role in cerebral injury.

Frequently Asked Questions

What are the advantages of using LPS injections in this model?

LPS injections induce stable inflammation, making them an effective method for studying CMHs and related processes in the brain.

How is the biological model implemented for studying CMHs?

The model involves administering LPS to Sprague-Dawley rats, followed by specific imaging and staining techniques to assess CMH presence.

What types of data are obtained from this method?

Key outcomes include visualization of CMHs, assessment of blood-brain barrier integrity, and molecular readouts from tissue staining.

In what ways can this method be adapted for other studies?

While focused on CMHs, the technique can also be applied to research on other brain disorders like depression and Alzheimer’s disease.

Are there any limitations or considerations when using this protocol?

Mortality rates can vary based on the age and health of the rats; thus, maintaining a clean environment is crucial to minimize risk.

Vi presentiamo un protocollo per indurre e rilevare CMHs causato da iniezione dei LPS in ratti Sprague-Dawley, che può essere utilizzato in futuro le indagini di ricerca sulla patogenesi di CMHs.

Questo metodo può aiutare a rispondere a domande chiave nel campo della micro-emorragia cerebrale come l'eziologia, la distribuzione e il rilevamento della micro-emorragia cerebrale e così via. Il principale vantaggio di questa tecnica è che le iniezioni di LPS inducono stabilmente infiammazione. Inoltre, l'iniezione LPS è abbastanza semplice, economica ed economica.

Sebbene questo metodo possa fornire informazioni sull'induzione dell'emorragia cerebrale, può anche essere applicato ad altri disturbi cerebrali come depressione, schizofrenia, morbo di Parkinson, morbo di Alzheimer e malattia dei prioni. Somministrare LPS a una dose di un milligrammo per chilogrammo di peso corporeo ai ratti maschi Sprague Dawley di 10 settimane per iniezione intraperitoneale, quindi riportare ogni topo nella gabbia di casa. Ripetere l'iniezione LPS dopo sei ore e 16 ore.

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