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How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
JoVE Journal
Biochimica
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JoVE Journal Biochimica
How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

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11:03 min

January 07, 2019

DOI:

11:03 min
January 07, 2019

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Summary

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Here, we present a protocol that involves genetically coupled spectrally distinct photoactivatable and fluorescent proteins. These fluorescent protein chimeras permit quantification of the PA-FP fraction that is photoactivated to be fluorescent, i.e., the photoactivation efficiency. The protocol reveals that different modes of photoactivation yield different photoactivation efficiencies.

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