JoVE Journal
Genetics
Genetics
This Contenuto is Open Access.
Capitoli
Riepilogo
We present a simple suppressor screen protocol in fission yeast. This method is efficient, mutagen-free, and selective for mutations that often occur at a single genomic locus. The protocol is suitable for isolating suppressors that alleviate growth defects in liquid culture that are caused by a mutation or a drug.