Un modello di cancro endometriale ortotopico con retroperitoneale Lmphadenopatia fatta da cellule VX2 in vivo propagate e coltivate

8K visualizzazioni

09:48 min.

12 settembre 2019

8K visualizzazioni

Lauren Philp¹^,² , Harley Chan³ , Marjan Rouzbahman⁴ , Ariana Rostami⁵ , Lili Ding⁶ , Scott V. Bratman⁵^,⁶ , Margarete K. Akens⁵^,⁷^,⁸ , Jonathan C. Irish⁷^,⁹^,¹¹ , Marcus Q. Bernardini¹⁰ , Gang Zheng⁵^,¹¹

¹Institute of Medical Science, University of Toronto, ²Department of Obstetrics and Gynecology, University of Toronto, ³Guided Therapeutics Laboratory, TECHNA Institute, University Health Network, ⁴Department of Pathology, Princess Margaret Cancer Center, University Health Network, ⁵Department of Medical Biophysics, University of Toronto, ⁶Princess Margaret Cancer Center, University Health Network, ⁷Techna Institute, University Health Network, ⁸Department of Surgery, University of Toronto, ⁹Department of Otolaryngology, Head and Neck Surgery, University of Toronto, ¹⁰Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center, University Health Network, ¹¹Princess Margaret Cancer Center, University Health Network

Questo protocollo presenta un metodo standardizzato per far crescere le cellule VX2 in coltura e per creare un modello ortotopico VX2 di cancro endometriale con metastasi di linfonodi retroperitoneali nei conigli. I modelli di cancro endometriali ortotopici sono importanti per lo studio preclinico di nuove modalità di imaging per la diagnosi delle metastasi dei linfonodi.