Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

Liang Wen; Wendong You; Yadong Wang; Yuanrun Zhu; Hao Wang; Xiaofeng Yang

doi:10.3791/59410

Indagare sulle alterazioni nel caecum Microbiota dopo una lesione cerebrale traumatica nei topi

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JoVE Journal Neuroscience

Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

Indagare sulle alterazioni nel caecum Microbiota dopo una lesione cerebrale traumatica nei topi

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04:29 min

September 19, 2019

DOI: 10.3791/59410-v

Liang Wen*¹, Wendong You*¹, Yadong Wang*¹, Yuanrun Zhu¹, Hao Wang¹, Xiaofeng Yang¹

¹Emergency and Trauma Center, The International Medical Center, The First Affiliated Hospital, School of Medicine,Zhejiang University

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Overview

This study presents a protocol for inducing diffuse traumatic brain injury (TBI) using a lateral fluid percussion device in mice. Following injury, the study investigates changes in gut microbiota by collecting caecum contents for analysis. The approach aims to elucidate the interactions between brain injury and gut microbiota composition.

Key Study Components

Area of Science

Neuroscience
Microbiome analysis
Traumatic brain injury research

Background

Understanding the relationships between the brain and gut microbiota is critical in TBI research.
Current knowledge of how TBI affects gut microbiota is limited.
The use of mice as a model organism allows for controlled experimental conditions.

Purpose of Study

To develop a reliable protocol for inducing TBI and analyzing subsequent changes in the gut microbiome.
To investigate potential correlations between neurological injury and gut microbiota diversity.
To provide insights into the impacts of TBI on overall health and microbiome interactions.

Methods Used

A lateral fluid percussion device was utilized to induce TBI in anesthetized mice.
Careful surgical procedures were undertaken, including skull penetration and cannula implantation.
Post-injury, caecum contents were collected for microbiome analysis and stored appropriately.
16S rDNA sequencing was employed to determine changes in microbiota diversity and composition.

Main Results

Analysis indicated reduced diversity in caecum microbiota within three days post-TBI.
Non-metric multidimensional scaling demonstrated significant shifts in microbiota composition following injury.
The study highlights the need for caution when handling tissues and maintaining sterile conditions.

Conclusions

This study establishes a methodology for exploring the gut-brain axis in the context of TBI.
Findings suggest that TBI may disrupt gut microbiota, which could have implications for recovery.
Enhancement of knowledge regarding gut-brain interactions could inform future therapeutic strategies for TBI.

Frequently Asked Questions

What are the advantages of using this TBI induction model?

The lateral fluid percussion method is relatively simple and efficient, facilitating controlled experiments to study TBI effects on the microbiome.

How is the brain injury induced in the study?

Anesthetized mice undergo cranial surgery, after which a lateral fluid percussion device administers a controlled injury.

What types of data are obtained from this study?

Data includes microbiome diversity and compositional changes analyzed through 16S rDNA sequencing.

How can this method be adapted for other research?

This protocol can be adapted to study other brain injuries or variations in microbiome analysis techniques.

What are key considerations when performing the surgical procedures?

Maintaining sterility and carefully manipulating tissues are crucial to avoid complications during the procedure.

Presentato qui è un protocollo per indurre lesioni cerebrali traumatiche diffuse utilizzando un dispositivo a percussione fluida laterale seguito dalla raccolta del contenuto di caecum per l'analisi del microbioma intestinale.

La simultaneità e le interazioni delle linee tra il cervello e il microbiota intestinale rimangono sconosciute. Utilizzare questa tecnica, è possibile esaminare le alterazioni del microbiota intestinale dopo lesioni cerebrali traumatiche nei topi. Il vantaggio principale di questa tecnica è che è relativamente semplice ed efficiente.

A dimostrare la procedura sarà Wendong You, uno studente laureato del mio laboratorio. Per l'induzione traumatica di lesioni cerebrali, confermare una mancanza di risposta al dito del piede in un topo anestetizzato e applicare un unguento agli occhi dell'animale. Dopo la rasatura, disinfettare la pelle esposta del cuoio capelluto con il 70% di etanolo prima di incisione del cuoio capelluto in un piano sagittale.

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