Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Ewa  M. Spiesz; Kui Yu; Benjamin  A.E. Lehner; Dominik  T. Schmieden; Marie-Eve Aubin-Tam; Anne S. Meyer

doi:10.3791/59477

Patterning tridimensionale di biofilm ingegnerizzati con Bioprinter fai da te

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Patterning tridimensionale di biofilm ingegnerizzati con Bioprinter fai da te

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08:40 min

May 16, 2019

DOI: 10.3791/59477-v

Ewa M. Spiesz*¹, Kui Yu*¹, Benjamin A.E. Lehner¹, Dominik T. Schmieden¹, Marie-Eve Aubin-Tam¹, Anne S. Meyer²

¹Department of Bionanoscience & Kavli Institute of Nanoscience,Delft University of Technology, ²Department of Biology,University of Rochester

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Overview

This article presents a method for transforming a low-cost commercial 3D printer into a bacterial 3D printer capable of printing patterned biofilms. It details the preparation of the bioprinter, bio-ink, and verification methods for assessing biofilm formation.

Key Study Components

Area of Science

3D Bioprinting
Microbiology
Bioengineering

Background

3D bioprinting with bacteria is an emerging technique.
This method allows for the construction of engineered biofilms.
It utilizes an inexpensive home-built 3D printer.
Applications include developing new antibacterial therapies.

Purpose of Study

To create reproducible model biofilms.
To facilitate the development of antibacterial therapies.
To demonstrate the versatility of 3D printing with various compatible bacteria.

Methods Used

Preparation of alginate-based bioink.
Calibration of the 3D printer's Z-axis.
Standard procedures for printing substrates.
Verification methods for biofilm formation.

Main Results

Successful printing of patterned biofilms.
Demonstrated compatibility with various bacteria.
Established reproducibility of model biofilms.
Provided a cost-effective solution for biofilm engineering.

Conclusions

The method enhances accessibility to 3D bioprinting technology.
It opens avenues for research in antibacterial therapies.
Further optimization of the printing process is encouraged.

Frequently Asked Questions

What is the main advantage of this 3D printing technique?

The main advantage is the ability to produce 3D printed biofilms using an inexpensive home-built 3D printer.

What types of bacteria can be used with this method?

Any type of bacteria that is compatible with the alginate-based bioink can be used.

What is the significance of the Z-axis calibration?

Calibrating the Z-axis is crucial for achieving accurate and successful 3D printing results.

How can the printed biofilms be utilized?

They can be used to develop new antibacterial therapies and study biofilm behavior.

Are the procedures for preparing bioink complex?

No, the preparation of the bioink and printing substrates are fairly standard procedures.

What verification methods are suggested for assessing biofilm formation?

The article describes various verification methods, though specific details are not provided in the summary.

In questo articolo viene descritto un metodo per trasformare una stampante 3D commerciale a basso costo in una stampante 3D batterica che può facilitare la stampa di biofilm a motivi geometrici. Sono descritti tutti gli aspetti necessari della preparazione del biostampa e del bio-Ink, nonché i metodi di verifica per valutare la formazione dei biofilm.

La biostampa 3D con batteri è una tecnica di nuova sviluppo. Questo protocollo fornisce un modo semplice per costruire biofilm ingegnerizzati stampati in 3D con batteri. Il principale vantaggio di questa tecnica è la capacità di produrre biofilm stampati in 3D utilizzando una stampante 3D costruita in casa economica.

Una possibile applicazione della nostra stampante 3D è quella di creare biofilm modello riproducibili che possono essere utilizzati per sviluppare nuove terapie antibatteriche. Il nostro approccio di stampa 3D può essere applicato a qualsiasi tipo di batterio compatibile con il nostro bioink a base di alginato. La preparazione del bioink e dei substrati di stampa sono procedure abbastanza standard, mentre il processo di stampa 3D, in particolare la calibrazione dell'asse Z è un passaggio cruciale che richiede una certa pratica.

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