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Immunology and Infection

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Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in Drosophila melanogaster

Article DOI: 10.3791/59510-v • 06:47 min • September 19th, 2019
September 19th, 2019 •
1, 2, 3, 4, 4
1NUS Environmental Research Institute & Department of Anatomy, National University of Singapore, 2NUS Environmental Research Institute & School of Public Health, National University of Singapore, 3Department of Civil and Environmental Engineering, National University of Singapore, 4Department of Anatomy, National University of Singapore

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We describe a detailed protocol for evaluating the toxicological profiles of zinc oxide nanoparticles (ZnO NPs) in particular, the type of cell death in human MRC5 lung fibroblasts and ROS formation in the fruit fly Drosophila.

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Toxicity Study Zinc Oxide Nanoparticles Cell Culture Drosophila Melanogaster Nanotoxicity Cell Death Reactive Oxygen Species Fluorescence Activated Cell Sorting DHE Probe Study Flow Cytometry High-content Imaging Zinc Oxide Nanoparticle Stock Solution Concentration MRC5 Human Lung Fibroblasts Incubation Treatment Centrifuge Phosphate-buffered Saline (PBS) FITC Annexin V Stain
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