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Immunology and Infection

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Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Article DOI: 10.3791/59543-v • 05:50 min • April 10th, 2019
April 10th, 2019 •
1,2, 2,3
1Department of Biology, University of Maryland, 2Department of Cell Biology and Molecular Genetics, University of Maryland, 3Institute for Bioscience and Biotechnology Research, University of Maryland

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This protocol describes an in vivo phagocytosis assay in adult Drosophila melanogaster to quantify phagocyte recognition and clearance of microbial infections.

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In Vivo Phagocytosis Assay Cellular Immune Response Fruit Fly (Drosophila Melanogaster) Genetic Screens Genome-wide Association Studies Novel Genes Phagocytosis Regulation Adult Blood Cells Pathogen Recognition Uptake Clearance Thin-wall Glass Capillaries Microscope Micrometer Needle Puller Fine-point Stainless-steel Tweezers Tip Diameter Liquid Volume Measurement Sterile 5% Food Coloring In PBS Mineral Oil Stage Micrometer Particles Injector Nozzle Fly Pad Anesthetized Flies Abdomen Injection
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