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Journal / Biochemistry / Expression and Purification of Nuclease-Free…
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Biochemistry

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JoVE Journal Biochemistry
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Article DOI: 10.3791/59599-v • 10:14 min • November 8th, 2019
November 8th, 2019 •
1, 1, 1, 1
1Cancer Biology and Genetics, College of Medicine, The Ohio State University

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Protocatechuate 3,4-dioxygenase (PCD) can enzymatically remove free diatomic oxygen from an aqueous system using its substrate protocatechuic acid (PCA). This protocol describes the expression, purification, and activity analysis of this oxygen scavenging enzyme.

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Nuclease-free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase PCD Expression Purification Nuclease Contaminants Transformation Reaction E.coli BL21 SOC Media LB-ampicillin Agar Incubation Culture Growth Optical Density Measurement
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