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Journal / Immunology and Infection / Pan-Lyssavirus real time RT-PCR voor rabiës…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Pan-Lyssavirus real time RT-PCR voor rabiës diagnose
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Pan-Lyssavirus real time RT-PCR voor rabiës diagnose

Article DOI: 10.3791/59709-v • 06:25 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1, 1, 1,2, 1, 1,2
1Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, 2Institute of Infection and Global Health, University of Liverpool

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Deze real-time RT-PCR met behulp van dsDNA intercalating Dye is geschikt om Lyssavirus infecties te diagnosticeren. De methode begint met RNA geëxtraheerd uit rabiës verdachte ante-mortem-of postmortemmonsters, detaillering Master Mix preparaat, RNA toevoeging, installatie van de real-time machine en juiste interpretatie van de resultaten.

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Immunologie en infectie uitgave 149 Lyssavirus rabiës real-time RT-PCR diagnose One-Step
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